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Why is Illumina’s Gene Expression (GEX) assay called Direct Hyb?

April 26, 2017assay called gene expression gex hyb illumina

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Why is Illumina’s Gene Expression (GEX) assay called Direct Hyb?

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Direct Hybridization (Direct Hyb) refers to the direct hybridization of probes on the array to the complementary cRNA molecule in your sample. This is in contrast to Illumina’s flagship GoldenGate® Genotyping Assay that incorporates a unique oligonucleotide onto the sample sequence. This unique oligonucleotide then hybridizes to the complementary probe on the array.