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Why is fragmentation buffer used instead of nebulization or sonication?

April 26, 2017buffer fragmentation nebulization sonication Used

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Why is fragmentation buffer used instead of nebulization or sonication?

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This fragmentation method takes advantage of the sensitivity of RNA to cleavage by divalent cations and temperature. This is the most robust method for fragmentation of RNA. Fragmentation by this method has been shown to result in more uniform sequencing coverage compared with other methods.