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Why is DNA polymerase added last during PCR?

April 26, 2017added dna PCR polymerase

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Why is DNA polymerase added last during PCR?

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DNA polymerase is an enzyme that finctions best when itis placed in the right ionic environment. All buffers and all other constituents required for the PCR reaction are added first. The buffers ensure the maintainace of the correct ionic environment for the polymerase to function optimally.