Why is distilled water used as blank in most of the colorimetric experiments?
Blank is not always prepared with distilled water,it is prepared basing on the solvent of stock solution, that is Eg:In glusoce estimation,for the stock solution preparation know amount of Glucose is dissolved in the water. For this experiment Blank is prepared with distilled water. Use of Blank reading: Blank reading gives only reagents concentration (without solute concentration).When we substract this blank reading from test readings,finally we get concentrating of solute.