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Why is a literature search required when writing a protocol?

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Why is a literature search required when writing a protocol?

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The PI must perform a literature search in order to ensure alternatives have be researched and considered for any aspect of the protocol that may cause more than momentary or slight pain or distress to the animal. Alternatives to be considered include those that would; 1. Replace animals with non animal alternatives 2. Refine the procedures to minimize discomfort that the animal(s) may experience 3. Reduce the number of animal used overall The literature search also helps to ensure the research proposal does not unnecessarily duplicate previous experiments.

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