Why I should prefer chromatogram files (SCF/ABI) over Fasta files?
DNA Baser Assembler is a tool like no other. One of its outstanding features is the capability to perform time consuming task (task that until now could only be done manually) instantly. For example by using DNA Baser Assembler you do not have to inspect your contig or manually correct the ambiguities. DNA Baser Assembler will do it for you. However, this feature works only if your input samples contain information about the quality of each base (QV or quality values). Therefore, whenever is possible you should use chromatogram files (SCF/ABI) files instead of FASTA files, empowering DNA Baser to automatically suggest corrections for the ambiguities. The accuracy of the suggestions can be as high as 95-99% and in most cases you don’t have to do any manual editing/corrections in your contig. This will save a lot of your precious time!!
