Why I am getting some pin point spotting in random places on the blot or dark artifacts in the blot?
• Pin point spotting can be related to bubbles or un-even wetting of the blot. • Larger dark artifacts in PVDF membrane sometimes are observed in places where the membrane was handled with the tweezers or that were inadequately wet with methanol. Do not handle PVDF membranes with tweezers until complete wet.
