Why heat-inactivate the serum used for MEF culture media?
While we heat-inactivate the serum in our MEF Medium, we do not heat-inactivate the serum in our hES Cryopreservation Medium. The decision to heat-inactivate may vary by lab philosophy. You can read about heat-inactivation of serum from Hyclone here: Heat Inactivation Note: Do not inactivate defined serum.