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Why have some predicted ORFs been cloned in both ORFeome version 1 and 3?

April 26, 2017cloned ORFeome orfs predicted version

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Why have some predicted ORFs been cloned in both ORFeome version 1 and 3?

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For most part, only ORFs that could not be cloned in ORFeome version 1 or that were cloned out of frame in ORFeome version 1, and that furthermore showed an updated exon/intron structure at the 5′ and/or 3’end between version WS9 and WS100 of Wormbase were part of the ORFeome version 3 dataset. We also attempted to clone a small number of ORFs that were already cloned in frame in ORFeome version 1 but for which new predictions exist.