Why has gluten come up positive but not wheat?
The wheat extract does not contain gluten but other proteins found in wheat such as seed storage proteins. The gluten extract does not contain these proteins but is a pure preparation of gluten. If you have antibodies to gluten but not to other proteins found in wheat, wheat will not show a positive result. Similarly, if you have antibodies to non-gluten proteins, wheat will show a positive result and in the absence of antibodies to gluten, gluten will remain negative.