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Why don’t you need to use the iodine dish when staining fecal smears prepared from specimens preserved in the newer single vial systems (zinc sulfate based PVA)?

April 26, 2017dish don’t fecal Iodine newer prepared preserved smears specimens staining

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Why don’t you need to use the iodine dish when staining fecal smears prepared from specimens preserved in the newer single vial systems (zinc sulfate based PVA)?

1 Answer

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The zinc sulfate-based PVA is water soluble, so the dry smears can be placed directly into the trichrome dish without having to go through the iodine and subsequent rinse steps. The zinc sulfate will be removed by the water in the trichrome stain.