Why don my 36 SNPs fit into 1 Sequenom pool?
In designing a multiplex assay, Sequenom design tools group together as many genotyping assays as it calculates are likely to be successful in combination, as some sequences can interfere with one another. The highest pooling efficiency occurs with large SNP lists-it would be extremely unlikely to ever group a list of 36 SNPs into 1 pool. If a pool of 36 SNPs was able to be designed there is always the possibility that some SNPs in the pool might not perform optimally when we try to multiplex 36 reactions.
