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Why does the hairpin insert sequence need to start with an adenine or guanine?

April 26, 2017adenine guanine hairpin insert sequence

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Why does the hairpin insert sequence need to start with an adenine or guanine?

1 Answer

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10 Posted

U6 and H1 are RNA Polymerase Type III promoters, which prefer a purine (adenine or guanine) for transcription. If the hairpin insert sequence does not start with an A or G, a G is added to the front of the hairpin insert sequence.