Why does the assay buffer supplied with topo II and gyrase sometimes go bad?
These buffers contain ATP which is somewhat unstable, particularly at the pH used in the 10x stock buffer. We cannot control this problem, however you should be aware that lack of strong topo II activity with positive controls (i.e. our enzymes), may be due to ATP degradation. In this case we recommend that you remake the buffer from the recipe given OR supplement with ATP.
