Why does K. lactis strain GG799 have no auxotrophic markers?
While auxotrophic markers have historically been used for genetic manipulation of yeasts, they are not always desirable to achieve maximum protein expression. In some cases, an auxotrophy (e.g. uracil) can cause a significant reduction in the strain’s ability to produce a heterologous protein even if exogenous uracil or uridine is provided in the growth medium.
