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Why does Epifluorescent microscopy work?

April 26, 2017microscopy

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Why does Epifluorescent microscopy work?

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A. Epifluorescent microscopy uses a UV-visible light source and specific filters to excite fluorescent stains added to microbiological specimens to aid in identifications and enumeration. Sterlitech Black Polycarbonate membranes are ideal for epifluorescent microscopy, since they retain bacteria on the surface of the membrane and provide a non-distracting background to view fluorescence against.