Why does CELLnTEC recommend to work without antibiotics / antimycotics for routine cultivation?
Primary cells are sensitive to the treatment with antibiotics / antimycotics. The supplementation of the medium with antibiotics / antimycotics can cause slower proliferation rates and can have influence on the differentiation behavior of cells. All commonly used antibiotics are not thermo-stable which can lead to the use of ineffective concentrations of antibiotics / antimycotics and thus enhance the risk of development of resistant microorganisms. For isolation of primary cells antibiotics / antimycotics are often needed because the tissue is not sterile, thus we recommend the use of antibiotics / antimycotics up to passage 2.
