Why do you recommend using SuperScript II instead of SuperScript III during the second round of amplification in the first-strand cDNA synthesis?
During the second round of amplification, using high temperature reverse transcription to achieve more full-length cDNA is not as critical as in the first round of amplification. Using a mesophilic reverse transcriptase such as SuperScript II generates higher yields of cDNA than with SuperScript III.
Related Questions
- Why do you recommend using SuperScript II instead of SuperScript III during the second round of amplification in the first-strand cDNA synthesis?
- Why do you recommend using SuperScript II and SuperScript III Reverse Transcriptases in the TargetAmp process?
- Can SuperScript III be used in place of SuperScript II?
