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Why do you recommend Hibernate-Ca without B27 for optimum dissociation with papain?

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Why do you recommend Hibernate-Ca without B27 for optimum dissociation with papain?

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Calcium promotes adhesion. Therefore, removing calcium promotes the dissociation of neurons. To offer cell adheasion proteins as the primary substrate for papain, B27 is ommitted so that proteins in B27 don’t compete for substrate.

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