Why do we use bovine serum albumin for plotting standard curve while doing protein estimation?
In reality all of these things boil down to practicalities in the end. BSA is readily available, of high quality and cheap in industrial quantities and has known biochemical properties. These qualities mean that it is easy to use. Also, you can readily compare data sets when they have been compared to the same standard, using the same protocols. This would be impossible if everyone used a different standard for doing essentially the same experiment. You could use anything as a standard if you knew the perfomance of that standard under a range of experimental conditions. Your first choice would never be a reactive enzyme such as DNAase as its kinetics could be hugely different under different experimental conditions (not to mention the difficulty of obtaining and maintaining it – enzymes are hugely expensive to buy) and there is no history of using human products as these are much harder to get hold of than animal ones for obvious reasons. History dictates many things in science, rabbit
