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Why do my qPCR amplification curves or plots decrease in fluorescence intensity after the saturation phase?

April 26, 2017amplification Curves fluorescence intensity phase plots qPCR saturation

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Why do my qPCR amplification curves or plots decrease in fluorescence intensity after the saturation phase?

1 Answer

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The optics of the qPCR instrumentation may be saturating due to improper instrument settings. Please consult with your instrument manufacturer for more details.