Why do my gDNA and cDNA/protein sequences need to be from the same genetic background?
The first step of TILLING is entering your gDNA and cDNA (or protein) sequences into CODDLe for analysis. If CODDLe detects any inconsistencies, or non co-linearity, you will get an error message and it will not proceed any further. A single nucleotide mismatch between the gDNA and cDNA will give an error. There can be slight variations in a gene’s sequence depending on its genetic background. This error can be corrected by changing the mismatched base(s) in either one of the sequences.
