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Why do I see amplification in a negative control DNA reaction with TempliPhi?

April 26, 2017amplification CONTROL dna negative REACTION templiphi

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Why do I see amplification in a negative control DNA reaction with TempliPhi?

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A. A. TempliPhi reactions are extremely sensitive to any traces of DNA. Primers present in the TempliPhi premix kits or any other contaminating DNA from other sources will amplify . Background Amplification is very inefficient and only occurs when very low or no DNA is added to reaction volume.