Why do I only get a very faint adenoviral band in the CsCl gradient?
This usually results from less than ideal amplification in the last round. If you infect 293 cells at too low titer (e.g. no obvious cell lysis after 4-5 days) or too high titer (e.g. cells lysed too soon, like less than 2 days), both will produce a faint band or no band on CsCl gradient preparations.
