Why do I need to use the single-stranded cDNA conversion factor when converting my A260 reading to cDNA concentration?
The amplified cDNA product of the WT-Ovation One-Direct System consists of both sense and antisense cDNA strands. While there may be some double stranded character to this mixture, we have developed and optimized One-Direct using the single stranded cDNA conversion factor (1 A260unit = 33 ng/µL of cDNA). Expected cDNA yield from amplification and input into labeling protocols cited in the product materials have all been generated using this convention.
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