Why do I need to confirm targeting in my expanded clones prior to blastocyst injections?
Potentially targeted clones identified in the initial screen are thawed, expanded, frozen and stored under stable conditions in LN2 dewars. It is important that these clones are confirmed by Southern as correctly targeted at both the 5’ and 3’ ends prior to microinjection. This ensures that the correct clones have been thawed and are of interest. It would be unfortunate to discover 6 months down the road that the mice you have been working with do not have the expected mutation. Additional DNA from the thawed/expanded clone(s) will be provided at this step.
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- Why do I need to confirm targeting in my expanded clones prior to blastocyst injections?
