Why do I have to label so much senseRNA for microarray hybridization?
In most RNA amplification methods, considerable quantities of amplified RNA are labeled for hybridization to microarrays. For example, Affymetrix requires 10µg of cRNA for hybridization to one GeneChip. Genisphere has compared sensitivity on microarrays using both amplified and unamplified RNA. Based on these experiments, we have made recommendations in our protocols for how much senseRNA to use in your labeling reactions.
