Why didn the Registry include some of their empty resistance plasmids in the DNA shipment?
While there were a few plasmid (backbones) shipped out, it’s true we haven’t included many bare plasmids. However, some the plasmids which we HAVE included instead are specialized construction plasmids designed to help you clone constructs into them in the following way: • Once you’ve transformed your plasmids into a cell, it’s hard to figure out which clones have actually been successful in acquiring the new DNA without checking individual colonies. • To aid you in selecting for these successful clones, we’ve included several plasmids with the BBa_P1010 BioBrick. Each plasmid features the part BBa_P1010 along with one or more resistance markers (Amp, Kan, Amp/Kan, Amp/Cm, Amp/Tet). This ccdB gene (BBa_P1010) is currently housed between the BioBrick restriction sites within a cell host (DB3.1) which tolerates the gene’s presence. However, should this plasmid containing the ccdB gene be transformed into a cell type of a different strain (ie. DH10B), the gene would cause cell death. • Th
