Why didn’t my cells grow after passaging them?
A very common problem researcher’s encounter when switching to a serum free or low serum medium is that the trypsin is not deactivated with the addition of medium. Over digestion with trypsin can irreversibly damage the cells, thus detachment must be closely monitored under the microscope. CELLnTEC recommends using a milder enzyme such as rProtease or Accutase for the passaging of cells.
