why consider digital imaging?
This article addresses how digital imaging performs under these conditions and what the advantages are of digital imaging for the optical microscopist. Illustrated in Figure 1 is a digital image of a thick section of mouse intestine stained with three fluorophores and recorded with a Nikon DXM1200 digital camera system coupled to the SMZ1500 stereomicroscope. The image was captured utilizing a combination of fluorescence and Nikon’s proprietary oblique coherent contrast (OCC) illumination techniques. A total of four integrations were performed and the image was composed in Photoshop to yield the final version presented in the figure. Why consider digital imaging? It is important to emphasize that the quality of the final image, whether digital or film, is dependent on the quality of the original microscope image. No matter how good the digital or conventional camera is, it cannot produce outstanding images from a poorly configured microscope. In addition, both film and digital imaging
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- why consider digital imaging?
