WHY CHOOSE THE METHYLAMP™ GLOBAL DNA METHYLATION QUANTIFICATION KIT?
• Very rapid procedure, which can be finished within 4 hours. • Colorimetric quantification without radioactivity, extraction, and chromatography. • Strip microplate format makes the assay flexible: manual or high throughput analysis. • Simple, reliable, and consistent assay conditions. Principle & Procedure The Methylamp™ Global DNA Methylation Quantification Kit contains all reagents required for quantification of global DNA methylation. In this assay, DNA is immobilized to the strip well specifically treated to have high affinity to the DNA. The methylated fraction of DNA can be recognized by 5-methylcytosine antibody and quantified through an ELISA-like reaction. The amount of methylated DNA is proportional to the OD intensity. SCHEMATIC PROCEDURE: Quantification of Methylated DNA. Product Components GM1 (10x wash buffer) GM2 (DNA binding solution) GM3 (methylated DNA control,100 µg/ml)* GM4 (block solution) GM5 (capture antibody, 1 mg/ml)* GM6 (detecting antibody, 100 µg/ml)* GM7
