WHY CHOOSE THE EPIQUIK™ TISSUE ACETYL-HISTONE H3 ChIP KIT?
• The fastest procedure available, which can be finished within 5 hours. • Strip microwell format makes the assay flexible: manual or high throughput. • Columns for DNA purification are included: save time and reduce labor. • Compatible with all DNA amplification-based approaches. • Simple, reliable, and consistent assay conditions. Principle & Procedure The EpiQuik™ Tissue Acetyl-Histone H3 ChIP kit contains all reagents required for carrying out a successful chromatin immunoprecipitation for acetyl-histone H3 from mammalian cells. Particularly, this kit includes a ChIP-grade acetyl-histone H3 antibody and a negative control normal mouse IgG. Chromatin from the cells is extracted, sheared and added into the microwell immobilized with the antibody. DNA is released from the antibody-captured acetyl-histone H3 protein-DNA complex, reversed and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications. SCHEMATIC PROCEDURE: Pro
