WHY CHOOSE THE EPIQUIK™ METHYL-HISTONE H3-K9 ChIP KIT?
• The fastest procedure available, which can be finished within 5 hours. • Strip microplate format makes the assay flexible: manual or high throughput. • Columns for DNA purification are included: save time and reduce labor. • Compatible with all DNA amplification-based approaches. • Simple, reliable, and consistent assay conditions. Principle & Procedure The EpiQuik™ Methyl-Histone H3-K9 ChIP Kit contains all reagents required for carrying out a successful chromatin immunoprecipitation for methyl-histone H3-K9 from mammalian cells. Particularly, this kit includes a ChIP-grade methyl-histone H3-K9 antibody and a negative control normal mouse IgG. Chromatin from the cells is extracted, sheared, and added into the microwell immobilized with the antibody. DNA is released from the antibody-captured methyl-histone H3-K9 protein-DNA complex, reversed and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications. SCHEMATIC PROCEDU
