WHY CHOOSE THE EPIQUIK™ GLOBAL DI-METHYL HISTONE H3-K9 QUANTIFICATION KIT (COLORIMETRIC)?
• Quick and efficient, which can be finished within 2.5 hours. • Innovative colorimetric assay with no need for radioactivity, electrophoresis, and chromatography. • Specifically capture di-methylated H3-K9 with the detection limit as low as 2 ng/well and detection range from 20 ng-5 µg/well of histone extracts. • The control is conveniently included for quantification of the amount of di-methylated H3-K9. • Strip microplate format makes the assay flexible: manual or high throughput. • Simple, reliable, and consistent assay conditions. Principle & Procedure The EpiQuik™ Global Di-Methyl Histone H3-K9 Quantification Kit (Colorimetric) is designed for measuring global histone H3-K9 di-methylation. In an assay with this kit, the di-methylated histone H3 at lysine 9 is captured to the strip wells coated with anti-dimethyl H3-K9 antibody. The captured di-methylated histone H3-K9 can be then detected with a labeled detection antibody followed by color development reagent. The ratio of di-met
