Why cells get stained with chemical dye/ stain?
The cells get stained because the colours adsorps to the membranes of cellular organelles and also to the cell wall. These adsorbed colours reflect light of their respective colours and we are able to see the colour of the stain. Generally, the cytoplasm doesn’t stain due to the lack of it’s adsorbrnt property with due exceptions. Staining is a biochemical technique of adding a class-specific (DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. It is similar to fluorescent tagging. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes. Stains may be used to define and examine bulk tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells, for instance), or organelles within individual cells. Biological staining is also used to mark cells in flow cyt