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Why can’t I use commercially available plastic backed precast gels with CyDye™ DIGE Fluor minimal dyes?

April 26, 2017cydye™ dige fluor gels plastic precast

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Why can’t I use commercially available plastic backed precast gels with CyDye™ DIGE Fluor minimal dyes?

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These gels come attached to a plastic backing, which generally has high background fluorescence. This inherent fluorescence of the plastic will significantly increase the background level, lower the dynamic range and reduce the number of protein spots seen in the gel.