Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

Why can i clone double stranded Oligo directly?

clone directly double oligo stranded
0
Posted

Why can i clone double stranded Oligo directly?

0 CommentsAdd a Comment

1 Answer

0

synthetic oligos do not have the 5′ phosphate group necessary for ligase to work. To clone directly you must add a 5′ phosphate using Polynucleotide Kinase(PNK) or order them nade with a chemical phophate added (at extra charge).

0 CommentsAdd a Comment

Related Questions

What is your question?

*Sadly, we had to bring back ads too. Hopefully more targeted.

Experts123