Why are the sample loading steps for the Mini and Midi spin columns extended to 6 min and 30 min respectively?
The metal chelate resin plug incorporates a technologically-advanced flow regulator which is designed to control the flow rate of the samples through the active column matrix. Observed yields and purities fluctuate as a direct function of the flow rate of the sample loading step. The flow regulator is pre-set by Pro-Chem to slow down the flow rate to an optimal capture speed. The concomitant increase in the residence time of the target protein with the matrix of the spin column increases substantially the yield and purity of the purified target protein. However, unlike many other chromatography systems, there are negligible hold-up volumes and His-tagged protein elution to recover the purified target protein is rapid for both Mini (1 min) and Midi (3 min) columns.
The metal chelate resin plug incorporates a technologically advanced flow regulator which is designed to control the flow rate of the samples through the active column matrix. Observed yields and purities fluctuate as a direct function of the flow rate of the sample loading step. The flow regulator is pre-set by AbD Seotec to slow down the flow rate to an optimal capture speed. The concomitant increase in the residence time of the target protein with the matrix of the spin column increases substantially the yield and purity of the purified target protein. However, unlike many other chromatography systems, there are negligible hold-up volumes and His-6-tagged protein elution to recover the purified target protein is rapid for both MINI (1 min) and MIDI (3 min) columns.
