Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

Why are the probes in the NCode miRNA probe set 40-44 bases long while the mature miRNAs are only 20-22 bases?

April 26, 2017bases Mature mirna mirnas probe probes

0

Posted

Why are the probes in the NCode miRNA probe set 40-44 bases long while the mature miRNAs are only 20-22 bases?

1 Answer

0

Posted

The probe set oligos are mature miRNAs synthesized as tandem repeats, so the same sequence is present in two adjacent copies (total length 40-44 bases). This helps in signal enhancement.