Why are the background intensity values higher than my signal intensity vales?
A. When ImaGene opens images for analysis, it reads information containded within the image file that explains which pixels represent high and low intensities. Users often assume that the visually white pixels always represent high values; however, this may or may not be the case depending on the information provided within the image file. The problem is that there are times when the image generation software does NOT include information describing what values white and black pixels should represent. The result is that ImaGene displays this information “backwards”, meaning what the user expects to contain high values in reality is being displayed with the inverse value. ImaGene provides a handy solution to this problem. On the Main User Interface, located under the Image Tab, is an Invert Checkbox. Select this checkbox to invert the value of pixels within the image. Visually, the image will “flip” causing high and low intensity pixels to change in the image window. Note: Selecting the
