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Why are the 50 mm glass bottom dishes useful for microinjection and maintaining a constant atmosphere in the dish?

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Why are the 50 mm glass bottom dishes useful for microinjection and maintaining a constant atmosphere in the dish?

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The 50 mm glass bottom dishes (part #’s beginning with P50G) are useful for: a) Microinjection: The larger diameter (50 mm) and the lower side wall (7 mm) allows easier access to cells in microinjection experiments. b) Atmosphere maintenance: The 50 mm dish has a cover that snaps onto the dish bottom and thereby prevents loss of the 5% CO2 atmosphere while the dish is out of the incubator. This can be important for experiments in which dishes will be observed for extended periods.

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