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Why are samples centrifuged at 4°C after the addition of chloroform when using RNeasy Lipid Tissue Kits?

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Why are samples centrifuged at 4°C after the addition of chloroform when using RNeasy Lipid Tissue Kits?

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Phase separation by centrifugation at 4°C after adding chloroform to the samples when using RNeasy Lipid Tissue Kits ensures minimizing genomic DNA contamination. Subsequent centrifugation steps …

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