Why are proteins labelled with minimal dyes (stable for 3 months at -70 C) more stable than those labelled with saturation dyes (stable for 1 month at -70 C)?
The shorter storage time recommended for CyDye™ DIGE Fluor saturation dyes is a precautionary measure due to differences in labelled protein concentration. CyDye DIGE Fluor saturation dyes are designed for use with scarce sample types and therefore, the concentration of the protein sample is likely to be relatively low (compared to samples suitable for use with CyDye DIGE Fluor minimal dyes). Proteins are less stable when stored at low concentrations, hence a shorter storage time is recommended when labelling with CyDye DIGE Fluor saturation dyes. The shorter storage time is not due to differences in stability between the two types of CyDye DIGE Fluor dye.
Related Questions
- Why are proteins labelled with minimal dyes (stable for 3 months at -70 C) more stable than those labelled with saturation dyes (stable for 1 month at -70 C)?
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