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Why are frosted plates not recommended for QPCR?

Frosted plates qPCR recommended
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10 Posted

Why are frosted plates not recommended for QPCR?

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10

Frosted plates have an uneven surface, causing uneven refraction of light and therefore high well-to-well variance. As consistency is a prerequisite to accuracy, variance should be kept to a minimum. Therefore frosted consumables are not recommended for QPCR.

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