Why and how did SCHOTT decide that the Nexterion® Slide NC-N/C nitrocellulose pads should be 11 microns thick?
There is a potential loss of fluorescence signal intensity from the labelled probes if they lie deep within the matrix of the nitrocellulose structure. It was determined empirically that 11 microns was the optimum thickness to use for nitrocellulose coated slides. To measure the effect of depth on signal intensity, 4 nL of 10µg/mL Cy-3 labelled anti mouse IgG was applied to a nitrocellulose membrane containing a light (photon) absorbing pigment. After spotting, analysis of the confocal signal intensity showed that fluorescence photons came from a maximum depth of 10 µm within the membrane. Fluorophors that lie below the surface do contribute to the overall signal, but with a reduced quantum yield the lower they are, due to light absorption within the membrane.
Related Questions
- SCHOTT has changed the name of the Nexterion® nitrocellulose slides, what are now the differences between the slides?
- Why and how did SCHOTT decide that the Nexterion® Slide NC-N/C nitrocellulose pads should be 11 microns thick?
- What is the thickness of the nitrocellulose membranes / pads on SCHOTT Nexterion® Slide NC-N/C?
