Why am I able to read wells containing the VIC™ dye when I am looking at the FAM dye layer?
Both VIC™ and FAM emit fluorescence in a similar range so that they will be visible in each others layers. If you are doing single dyes in a well you should not label the wells with an inappropriate dye. If you are multiplexing the two dyes together make sure that you have the spectral compensation for real-time turned on. This will tighten the range and filter out the wavelengths that are overlapping. Multiplex assay design procedures are covered in User Bulletin #5 which can be downloaded from our website.
