Which SUPERSOMES™ should I use…plus or minus cytochrome b5?
Customers have the choice in using SUPERSOMES™ containing cDNA-expressed cytochrome b5. The incorporation of cytochrome b5 into several P450 SUPERSOMES™ products increases the respective cytochrome P450 activity (insect cell microsomes do not contain significant amounts of endogenous cytochrome b5). These b5 containing microsomes are particularly useful for our high throughput P450 inhibition screening method where the turnover of the fluorescent substrate can be increased several fold. However, increases in substrate turnover is substrate specific. For example, there is little change in CYP2C9*1 diclofenac 4’-hydroxylase activity in the presence of b5.
