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Which strand of DNA is packaged when cells carrying the pMAL vectors are superinfected with an M13 helper phage such as M13KO7 (NEB cat #315)?

April 26, 2017carrying cells dna Helper m13 pmal strand vectors

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Which strand of DNA is packaged when cells carrying the pMAL vectors are superinfected with an M13 helper phage such as M13KO7 (NEB cat #315)?

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The strand corresponding to the antisense strand of the malE gene is packaged. This is the strand that is complementary to the polylinker sequence shown in the 1998/99 catalogue on p. 236. The malE primer (#S1237S) hybridizes to the strand that is packaged by the helper phage.