Which species to chose for immunisation?
Advantages of using both IgG (rabbit, goat) and IgY (hen) antibodies developed against the same antigen: • independent confirmation that expected target protein is detected • antibody pools with distinct properties, complementing each other in different techniques (Western Blot, immunoprecipitation etc.) • double staining possible Generaly for immunisations choose an animal genetically distant from the antigen source (e.g. hens are very suitable for production of antibodies against conserved mammalian proteins). On this page (IgY) you will find more information about antibodies in hens.
