Which reagents are compatible with the Strep-tag®/Strep-Tactin® interaction?
Reagent Concentration Reduction Agents DTT β-mercaptoethanol Non-Ionic Detergents C8E4; Octyltetraoxyethylene max. 0.88 % C10E5; Decylpentaoxyethylene 0.12 % C10E6 0.03 % C12E8 0.005 % C12E9; Dodecyl nonaoxyethylene (Thesit) 0.023 % DM; Decyl-β-D-maltoside 0.35 % LM; N-dodecyl-β-D-maltoside 0.007 % NG; N-nonyl-β-D-glucopyranoside 0.2 % OG; N-octyl-β-D-glucopyranoside 2.34 % TX; Triton X-100 2 % Tween 20 2 % Ionic Detergents N-lauryl-sarcosine 2 % 8-HESO;N-octyl-2-hydroxy-ethylsulfoxide 1.32 % SDS; Sodium-N-dodecyl sulfate 0.1 % Zwitter-Ionic Detergents CHAPS 0.1 % DDAO; N-decyl-N,N-dimethylamine-N-oxide 0.034 % LDAO; N-dodecyl-N,N-dimethylamine-N-oxide 0.13 % Others Others Ammonium sulfate (NH4)2SO4 2 M CaCl2 max. 1 M EDTA 50 mM Ethanol 10 % Guanidine max. 1 M Glycerol max. 25 % Imidazole max. 250 mM MgCl2 1 M NaCl 5 M Urea max. 1 M Note: These reagents have been successfully tested for the purification of e.g. GAPDH-Strep-tag with concentrations up to those mentioned. For reagents not
